Bridget Hua Bui L&S Sciences
Identification of Anti-CRISPRs for Genome Editing Safety
Since the discovery of the CRISPR-Cas adaptive immune system in prokaryotes, Cas9 proteins have been used as a genome editing tool in a wide variety of organisms and promise to provide new solutions to long-standing, unsolved problems in human disease. However, despite the great potential CRISPR technology holds, concerns remain about the risk of unwanted edits in the human genome that could lead to genetic disease. Therefore, my project aims to find anti-CRISPR proteins that serve as off-switches for Cas9 activity to ensure gene editing safety and prevent malicious use of this tool. To achieve my goal, I will first identify potential anti-CRISPR candidates using a newly developed bioinformatics pipeline and then experimentally screen these candidates for anti-CRIPSR activity.