Daniel Polyakov Rose Hills
Determining the Regulatory Genes Involved in Interspecies Interactions
Due to the overuse of antibiotics, bacterial resistance has become one of the most widespread epidemics facing the world today. This, combined with a significant decrease in the production of antibiotics has created a need for new drugs to combat bacterial infections. Actinomycetes are a class of bacteria known to produce several secondary metabolites important in modern medicine. Recent innovations in Next Generation Sequencing have proved that Actinomycetes are capable of producing far more metabolites than previously thought possible. However, the mechanism through which these bacteria are prompted to produce these special metabolites is unknown. My project this summer is to identify the essential genes involved in bacterial communications through secondary metabolites. I aim to do this through with a genetic screen of the bacteria Streptomyces ceolicolor during an interspecies interaction. Then, using modern biological technologies such as PCR, transformation, and metabolomics I will be able to determine the essential genes and their functions.