David Noble Rose Hills
Elucidating Genetic Interaction Networks of Post-Transcriptional Regulators in Yeast
Post-transcriptional regulation is vital for cell survival and proliferation in diverse environments, but little is understood about the underlying regulatory networks or their mechanisms. Regulation of mRNA expression is an essential process in cells that involves RNA binding proteins targeting mRNAs to change their localization, expression, and stability. These RNA binding proteins do not act in isolation; they are often involved in various pathways that also regulate their function. Our lab group has designed and performed numerous high-throughput screens to collect data about genes and proteins involved in post-transcriptional regulation on the genomic level in Saccharomyces cerevisiae. One such screen is a CRISPR interference (CRISPRi) screen, which inactivates genes on a genome-wide scale to reveal how each target gene affects a specific biological process, such as the activity of a particular RNA binding protein. The goal of my project is to synthesize the data from these high-throughput screens, formulate a valid measure for effect on translation, and ultimately elucidate the mechanisms, functions, and regulation of various genes on post-transcriptional regulation.