Dawei Liu L&S Sciences
Treating Huntington's Disease by Autophagy
Huntingtons disease (HD) is a neurodegenerative disease resulting from accumulation of neurotoxic protein aggregates in the brain. The leading cause is the abnormal expansion of glutamine (polyQ) in the huntingtin protein (HTT). A major quest in this field is to develop strategies to clear the polyQ HTT aggregates. Autophagy is an essential pathway for cellular homeostasis by removing damaged proteins and organelles through the formation of autophagic membranes. The aim of my project is to manipulate autophagy to enhance the clearance of polyQ HTT aggregate. Specifically, I will design a series of protein adaptors that can theoretically enhance the targeting of the autophagic membrane to the polyQ HTT aggregate and test their effect on autophagy-mediated clearance of polyQ HTT aggregates. My methodological approaches include cloning, flow cytometry, immunoblot, co-immunoprecipitation, and immunofluorescence.