Hanna Liao Rose Hills
Investigating the degradation of a kinetochore protein, Ndc80p, during meiosis
Sexual reproduction in almost all known organisms requires a specialized cell division named meiosis. Errors in this cell division are the leading cause of genetic disorders such as Downs syndrome, miscarriages, and congenital birth defects in humans. To ensure the success of meiosis, chromosomes undergo a dynamic restructuring of their kinetochores, which mediate chromosome segregation. In budding yeast, a single kinetochore subunit named Ndc80p is down-regulated in meiotic prophase to ensure proper meiotic chromosome segregation. Preliminary results by our lab have shown that Ndc80p degradation depends on the proteasome, potentially suggesting an ubiquitin-mediated degradation mechanism. Since the target specificity of this system requires E3 ubiquitin ligases, I aim to screen a subset of known or predicted E3 ligases to identify the one(s) that targets Ndc80p for degradation. Identifying such E3 ligase(s) and studying its regulations in meiosis will contribute to a mechanistic understanding on how cells regulate kinetochore composition to ensure proper meiosis.