Jane O L&S Sciences
Screening for Silence: Bypassing Sir2 deacetylation in yeast
Because any given cell carries an overwhelming excess of genetic information, they need a way to selectively pinpoint what they need, when they need it. Silencing entire regions of the genome via chromatin modifications is one method that eukaryotes have developed to great effect. The four SIR (Silent Information Regulator) genes are absolutely critical to silencing in yeast. Wild-type Sir3 typically requires Sir2s deacetylation activity, and not just the deacetylation itself, in a mechanism that is still unclear. I will use sequencing and protein structural analysis to study PCR generated point mutations in the SIR3 gene that recover silencing in order to help me identify key functional regions in the Sir3 protein.