Jillian Lee Silvestrini L&S Sciences
Investigating CRPYTOCHROME 2 (CRY2) and EARLY FLOWERING 3 (ELF3) as molecular partners in the circadian system in Arabidopsis thaliana
I am studying the possible physical or functional interaction between ELF3 and CRY2, two genes in Arabidopsis thaliana that are involved in regulating the plants biological clock. I will study ELF3 and CRY2 using Arabidopsis plants that have mutations in these genes, resulting in dysfunctional proteins. Many of the phenotypes of the single mutants are opposite, which helps to facilitate analysis of the gene interaction. I will look at flowering time, monochromatic and white light signaling (using hypocotyl elongation as an assay), and regulation of 24-hour rhythms using Luciferase as a reporter gene. I will also analyze the protein products on a molecular level, using yeast two-hybrid and co-immuno-precipitation experiments. The interaction of these two genes could help reveal the biological pathways involved in light signaling and floral induction.