Katie Shalvarjian Rose Hills
Genome-Scale Fitness Profiling of Ralstonia Solancearum in Xylem Sap
Ralstonia solanacearum is a unique vascular plant pathogen that thrives in xylem sap. As a nutrient-poor but chemically complex environment, xylem sap provides distinct obstacles to bacterial growth, yet little is known about R. solanacearum within the framework of genome-wide fitness. Using a randomly barcoded transposon site sequencing (RB-TnSeq) mutant library, we can measure gene-specific fitness contributions to R. solanacearum in xylem sap, along with various growth media, to understand what genes drive its fitness in a natural context. As a causal factor of bacterial wilta disease that wipes out crops of small shareholder farmersit is important to understand R. solanacearum within the context of its natural environment in order to accurately represent its pathogenicity in planta. This study aims to develop a genetic understanding of R. solanacearum virulence in xylem sap, providing important information that can be used to develop a holistic understanding of its growth and spread.