Katrina Olson Rose Hills
How is gene expression controlled during development? Identifying minimal promoters in Ciona Intestinalis
Proper gene expression relies on the precise communication between promoters and enhancers. It is not well understood how promoters and enhancers work to ensure the expression of the right genes at the right time. I plan to create minimal functional promoters in order to find the smallest unit of the promoter capable of driving gene expression in Ciona intestinalis, a sea squirt. Currently, the promoters used are longer than the core functional promoter and therefore may contain other, unknown information. With a minimal promoter of about 100 base pairs, it will be easier to analyze the necessary and conserved sequences in the promoter that allow the promoter and enhancer to communicate.