Nan “Chuck” Ye Rose Hills
Efficient bioconjugation through UV-activated oxidative coupling of azidophenol and Aniline
Bioconjugation reactions, the chemical reactions connecting two biomolecules together, have been heavily studied to chemically modify proteins, allowing the possibility of augmenting the biological activity of proteins with small molecules. This field of research is crucial to many pharmaceutical applications, such as drug targeting that requires the conjugation between drug molecules and soluble proteins. In order to install new synthetic molecules onto a protein site specifically and bioorthogonally, there is an increasing need for chemical strategies that can effectively attach synthetic molecules site specifically to proteins. However, many of these reactions often require high excess of reagents, and the types of reactions that can selectively target amino acids are limited. My project is to investigate the scope of bioconjugation reaction by studying the UV-light activated oxidative coupling reaction between azidophenol and anline. The research project will potentially lead to new bioconjugation method with high yields, chemoselectivity and expanded conjugation substrates.