Parker Stow Rose Hills
Cycloprodigiosin Enzyme Discovery: Searching for the Gene Implicated in the Biosynthesis of a Novel Marine Natural Product
Cycloprodigiosin is a novel chemical compound produced by a number of marine bacterial species. One such species of cycloprodigiosin producing bacteria, Pseudoalteromonas rubra or P. rubra, is convenient in that it has had its genome fully sequenced. The gene(s) responsible for the synthesis of cycloprodigiosin from its precursor prodigiosin, however, are yet still unknown and have eluded researchers to date.In this project I will comb through the P. rubra genomic library in search of the enzyme(s) of interest. Gene knockout studies will validate which enzymes are responsible for the synthesis of cycloprodigiosin and further work could then be conducted to characterize any enzymes of interest.