Pritika Acharya L&S Biological Sciences
Optimizing Single-Cell Patterning for Breast Cancer and Immune Cells
Breast cancer is the second leading cause of cancer deaths in the United States. Triple-negative breast cancer (TNBC) is a subtype that accounts for approximately 20% of all cases and is difficult to treat due to the lack of targeted therapies. The immune system is highly integrated with the breast cancer microenvironment, and macrophages play a large role in breast cancer progression. I am interested in studying the interplay between breast cancer cells and macrophages using high throughput DNA-directed patterning (htDNA-dp). This platform allows for the selective patterning of single cells in a controlled manner. I plan on optimizing the platform to pattern single-cell breast cancer cells and macrophages to eventually study the interactions of these cell types on a single-cell level. Once the platform is optimized, I will validate my cell lines using immunofluorescence staining for various surface markers to inform the identity of different cells for future experiments.