Shea Khyeam L&S Sciences
In Vitro Analysis of Cardiomyocyte Binucleation in H9C2 Cell Line
Although animals such as zebrafish and newborn mice retain the ability to regenerate the heart post-injury, adult mammalians have largely lost this cardiac regenerative capacity. Consequently, a patient will irreversibly lose as many as a billion cardiomyocytes following a heart attack and suffer from permanently reduced cardiac function. Today, nearly five million Americans live with heart failurethis underscores the significance of our inability to regenerate myocardial tissue.
Most mammalian cardiomyocytes lose their proliferative and regenerative abilities because they undergo binucleation, terminal differentiation, and permanent withdrawal from the cell cycle postnatally. The mechanisms which control this transition, however, are still not fully understood. In fact, despite the amount of in vivo research, there is no in vitro model that has been comprehensively used to analyze endogenous regenerative mechanisms that partake in cardiomyocyte cell cycle arrest and binucleation. Thus, I plan to work with the H9C2 cell line to try to reproduce in vivo results, study cardiomyocyte binucleation mechanistically, and to establish whether it may be a viable in vitro model to study cardiac regeneration.