Siddharth Chittaranjan L&S Sciences
Comparative Dual RNA-Seq Transcriptome Profiling of Host-Pathogen Interactions During Chlamydia Trachomatis Infection of Human Primary Endocervical Cells
Microbes pose a wide spectrum of nonpathogenic and pathogenic challenges for the immune system. The encounters are governed by the interactions between the bacteriums adaptations and the hosts ability to mount a protective immune response. Chlamydia trachomatis (Ct) is an especially unique pathogen as the infections it causes in the genital tract are typically asymptomatic. While this suggests that Ct possesses an effective set of tools to cloak itself as a lesser threat to the immune system, these tools still vary in success, as Ct strains elicit different immune responses depending on the cell type the bacterium infects. Hence, I propose to use a new technology called dual RNA-seq to analyze how human primary cells from patients vary in their response to infection with Ct and determine factors that distinguish successful immune responses from unsuccessful ones. Overall, by tracking Ct and host genes simultaneously, this data could lead to preventative strategies by giving us the chance to interrogate the disease-causing mechanisms of Ct.