Wing Shing Yip L&S Sciences
Exploring the Difference between Thermophilic and Mesophilic Proteins Using Protein Engineering
T. thermophilus RNase H (TthRNase H) is a protein that is stable at high temperatures. In my project, I will examine how the amino acid sequence of TthRNase H determines its folding properties that lead to its thermostability. To approach this question, I am going to construct two proteins, one containing the core of C. tepidum RNase H (a protein stable at moderate temperatures) and the periphery of TthRNase H and another containing the core of C. tepidum RNase H and the the periphery of E. coli RNase H (a protein stable only at low temperatures). Using CD spectroscopy to analyze these proteins, I hope I will be able to get more insights on folding mechanisms of proteins.