Xammy Nguyenla Rose Hills
Identification of IFN-inducible Genes Essential in Control of Tuberculosis Infection Using a CRISPR/Cas9 System
IFN- is a cytokine that has essential immunostimulatory and modulatory roles in innate and adaptive immune responses. More specifically it is released by host CD4+ T cells and has been shown to be essential in macrophage-mediated control of Mycobacterium tuberculosis. Many downstream pathways have been proposed, including ones involving inducible nitric oxide synthase (iNOS), autophagy induction, and GTPase activity; however, some models are contradictory and others unclear, leaving IFN-dependent immunity against M.tb poorly understood. Previous RNASeq. data from the Stanley Lab identified a pool of genes upregulated by IFN- in macrophages infected with M.tb. With this as a foundation, we will use a CRISPR-Cas9 system to knock out and screen for prospect IFN-inducible genes in primary macrophages that are important for control of M.tb.