Spleen Development in TLx1 and NKx2.5 deficient human cell lines
TLx1 and Nkx2.5 are transcription factors that are essential regulators of early spleen development. In order to better understand how conditions such as incomplete congenital asplenia, and other aberrations of the spleen develop, better understanding the effects of these transcription factors on development is necessary. In order to do so I am generating spleen cell lines from both human fetuses and mouse embryos that are deficient in Tlx1 and Nkx2.5 by conducting genome editing through the CRISPR-cas9 platform. Upon achieving this I will be able to understand specifically how these transcription factors function and their relationship to congenital disease.