Finding Novel Regulators of Nonsense-mediated mRNA Decay
Nonsense-mediated mRNA decay (NMD) is a post-transcriptional regulatory pathway that degrades transcripts containing premature termination codons. The pathway plays an important role in eukaryotic gene regulation, with hundreds to thousands of transcript targets across diverse species, including important regulators of developmental and stress response pathways. Over the past year, I have worked under the supervision of postdoctoral researcher Zhiqiang Hu to develop methods for using genomic data, including RNA-sequencing data previously collected by the lab and publicly-available sequencing data from ENCODE, in order to identify novel regulatory proteins involved in the NMD pathway. My work during the summer will build upon this by using these methods to identify novel regulators of NMD, as well as find associations between binding sites of these regulators to infer possible mechanisms and relationships between regulators.