Immunohistochemical Analysis of nNos+ Amacrine Cells in Mouse Retina
My research specifically hones in on the functionality and morphology of amacrine cells. Amacrine cells can be GABAergic, glycinergic, or neither depending on what inhibitory neurotransmitter they express (GABA, glycine, or neither). While both the GABAergic and the glycinergic amacrine cells have been fairly well studied, there is not much extensively known about the 15% of amacrine cells that fall under the neither category. To address this lack of information on the neither type cell, my part within the larger research project is performing immunohistochemical stains on the tissue of mouse retina. I stain for various markers, specific either to amacrine cells, the bipolar cells it communicates with, or general synaptic markers. These stains indicate the location of the amacrine cells within the layers of the retina. By determining the location of the amacrine cell, or where its dendrites arborize in the inner plexiform layer, it can be precisely determined which other cells the “neither” amacrine cell tends to speak to. This type of location specificity and morphological evidence gives us a chance to interpret and then extrapolate the specific functionality of these neither amacrine cells within the retina.