Producing Stable Transformants in Maize using Proprietary Transformation Vectors
This summer I will be continuing my two years of effort to produce a stable maize transformant. I will be using a proprietary strain of agrobacteria, a bacteria that inserts its genome into that of plants to insert my genes of choice into embryos of maize and seteria. This process does not provide reliable transformants yet, so I will be testing how the plant’s genotype affects efficiency of transformation, as well as the agrobacterium genotype and effects of transformation helper plasmids. Additionally, I will be genotyping my transformants and observing their phenotypes using techniques such as tissue sectioning and staining and fluorescence microscopy.